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Semen Specimen Collection

The semen collection procedure is to be used for either a complete semen analysis or a post-vasectomy screen.


At least six ejaculations are needed for spermatozoa to clear from the semen after vasectomy. Collect the specimen after this period of time as instructed by the doctor.  The collection container should be a clean wide-mouth glass jar or a sterile specimen container. 


A 2-4 day abstinence period is necessary for this test.  The Seminal Fluid Collection kit supplied by the laboratory should be used. 


1. The specimen can be obtained by masturbation and ejaculated directly into the specimen container. Collect the entire specimen avoiding spillage. If any of the specimen is spilled, label the sample container as incomplete specimen.

2. The sample can be obtained through intercourse if the non-spermicidal polyurethrane condom provided in the Seminal Fluid Collecting Kit is used. Other condoms are not acceptable because they often contain spermicidal lubricants and powders. Note: Contraceptive solutions and lubricants cannot be used.

The sample must be maintained at a temperature between room and body temperature until it is submitted. Keep the sample close to the body to keep it warm. Label the specimen container with patient's name, date of birth, and date and time of collection.


YORK HOSPITAL - The specimen must be delivered to the Lab Collection Center at York Hospital within one hour after collection between the hours of 6:30 a.m. and 9:30 a.m. Monday through Friday.  Specimens are not accepted on weekends or holidays.  If necessary, special arrangements can be made with the lab to accept specimens on a Saturday morning by calling (717) 851-2579.  Patients may register with Patient Registration in the Atrium at York Hospital. 

GETTYSBURG HOSPITAL - The testing must be scheduled by calling (717) 337-4120, ext. 75187.  The specimen must be delivered to the Out-patient Laboratory at Gettysburg Hospital within thirty minutes after collection between the hours of 7:30 a.m. and 10 a.m., Monday throughFriday.  Specimens are not accepted on weekends or holidays.  If necessary, special arrangements can be made with the lab to accept Post Vasectomy specimens only on a Saturday morning.

GOOD SAMARITAN HOSPITAL - Post vasectomy specimens can be received in the lab between 6:30 AM and 2 PM Monday through Friday.

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